Journal: eLife
Article Title: High-intensity interval training remodels the proteome and acetylome of human skeletal muscle
doi: 10.7554/elife.69802
Figure Lengend Snippet: Figure 5. HIIT increases acetylation of mitochondrial and TCA cycle proteins concomitantly with an increase in SIRT3 abundance. (A) Volcano plot displaying 20 upregulated (filled red circles) and 1 downregulated (filled blue circle) acetyl-sites following HIIT at an FDR <0.05, while 257 acetyl-sites were upregulated (red circles) and 26 downregulated (blue circles) at Π<0.05 (n=7). (B). Scatter plot indicating that HIIT-induced changes in acetyl-site intensity typically exceeded that of the corresponding protein. (C). Fisher’s exact tests identified the enrichment of mitochondrial and TCA cycle terms
Article Snippet: Acetyllysine: #9441, Cell Signaling Technologies; EP300: #sc32244 (human skeletal muscle); #sc48343 (C2C12s), Santa Cruz Biotechnology, 300 kDa; ac- H2B K20: #ab177430, Abcam, 14 kDa; H2B: #12364, Cell Signaling Technologies, 14 kDa; ac- H3 K23: #14932, Cell Signaling Technologies, 17 kDa; H3: #4499, Cell Signaling Technologies, 17 kDa; MYLK2: PA5- 29324, Invitrogen, 65 kDa; OXPHOS antibody cocktail: ab110411, Abcam, 15–55 kDa; SIRT1: 07–131, Millipore, 80 kDa; SIRT3: #5490, Cell Signaling Technologies, 28 kDa; SIRT5: #8782, Cell Signaling Technologies, 30 kDa; SIRT6: #12486, Cell Signaling Technologies, 36–42 kDa; ac- SOD2 K68: #ab13737, Abcam, 24 kDa; ac- SOD2 K122: #ab214675, Abcam, 24 kDa.
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